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, that's the more frequent kind of HPLC, the stationary phase is nonpolar as well as the cellular stage is polar. The most typical nonpolar stationary phases use an organochlorosilane in which the R group can be an nChiral chromatography is used when the separation of enantiomers is essential. Enantiomers hold the similar Actual physical and chemic
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And although likely again to the bottom condition, it can demonstrate the fluorescence and that is then detected because of the photocell. Every time a compound is not By natural means fluorescent, the fluorescent dye is used to detect the emission. Examples of fluorescent dyes: Dansyl chloride for amino acids and phenols for protein hydrolysates.A
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